m059k cell line Search Results


m059k (dna-pk +/+ ) cell line  (Coriell Institute for Medical Research)
90
Coriell Institute for Medical Research m059k (dna-pk +/+ ) cell line
Loss of DNA-PK, ATM and expression of a kinase dead (kd) ATR renders cells sensitive to UV radiation. <t>M059K</t> (DNA-PK +/+ ), M059J (DNA-PK −/− ), GM02184E (ATM +/+ ), GM03189D (ATM −/− ), GM847 (ATR +/+ ) and GM847Kd (ATR kinase dead) were exposed to 50 or 100 J/m 2 UV and analyzed for DNA content after ( A ) 24 and ( B ) 48 hours. The percentage of cells with hypodiploid DNA content is indicated.
M059k (Dna Pk +/+ ) Cell Line, supplied by Coriell Institute for Medical Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m059k (dna-pk +/+ ) cell line/product/Coriell Institute for Medical Research
Average 90 stars, based on 1 article reviews
m059k (dna-pk +/+ ) cell line - by Bioz Stars, 2026-03
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Loss of DNA-PK, ATM and expression of a kinase dead (kd) ATR renders cells sensitive to UV radiation. M059K (DNA-PK +/+ ), M059J (DNA-PK −/− ), GM02184E (ATM +/+ ), GM03189D (ATM −/− ), GM847 (ATR +/+ ) and GM847Kd (ATR kinase dead) were exposed to 50 or 100 J/m 2 UV and analyzed for DNA content after ( A ) 24 and ( B ) 48 hours. The percentage of cells with hypodiploid DNA content is indicated.

Journal: OncoTargets and therapy

Article Title: Dynamic changes to survivin subcellular localization are initiated by DNA damage

doi:

Figure Lengend Snippet: Loss of DNA-PK, ATM and expression of a kinase dead (kd) ATR renders cells sensitive to UV radiation. M059K (DNA-PK +/+ ), M059J (DNA-PK −/− ), GM02184E (ATM +/+ ), GM03189D (ATM −/− ), GM847 (ATR +/+ ) and GM847Kd (ATR kinase dead) were exposed to 50 or 100 J/m 2 UV and analyzed for DNA content after ( A ) 24 and ( B ) 48 hours. The percentage of cells with hypodiploid DNA content is indicated.

Article Snippet: Osteosarcoma (SaOS2), breast carcinoma (MCF7) cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA) while M059K (DNA-PK +/+ ), M059J (DNA-PK −/− ), GM02184E (ATM +/+ ), GM03189D (ATM −/− ) cells were obtained from the Coriell Institute (Camden, NJ).

Techniques: Expressing

Survivin, p53 and p21, after UV radiation, are differentially expressed in PI3K related kinase competent versus incompetent cells. A ) M059K (DNA-PK +/+ ), M059J (DNA-PK −/− ), B ) GM02184E (ATM +/+ ), GM03189D (ATM −/− ), C ) GM847 (ATR +/+ ) and GM847Kd (ATR kinase dead) were exposed to 50 or 100 J/m 2 UV and analyzed by Western blotting after 24, 48, and 72 hours.

Journal: OncoTargets and therapy

Article Title: Dynamic changes to survivin subcellular localization are initiated by DNA damage

doi:

Figure Lengend Snippet: Survivin, p53 and p21, after UV radiation, are differentially expressed in PI3K related kinase competent versus incompetent cells. A ) M059K (DNA-PK +/+ ), M059J (DNA-PK −/− ), B ) GM02184E (ATM +/+ ), GM03189D (ATM −/− ), C ) GM847 (ATR +/+ ) and GM847Kd (ATR kinase dead) were exposed to 50 or 100 J/m 2 UV and analyzed by Western blotting after 24, 48, and 72 hours.

Article Snippet: Osteosarcoma (SaOS2), breast carcinoma (MCF7) cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA) while M059K (DNA-PK +/+ ), M059J (DNA-PK −/− ), GM02184E (ATM +/+ ), GM03189D (ATM −/− ) cells were obtained from the Coriell Institute (Camden, NJ).

Techniques: Western Blot

UV radiation repartitions survivin from the cytosol to the mitochondria and nucleus. M059K (DNA-PK +/+ ), M059J (DNA-PK −/− ), GM02184E (ATM +/+ ), GM03189D (ATM −/− ), GM847 (ATR +/+ ) and GM847Kd (ATR kinase dead), exposed to 50 or 100 J/m 2 UV for 24 hours were subcellularly fractionated into cytosol ( A ), mitochondria ( B ) and nuclear ( C ) fractions and analyzed by Western blotting.

Journal: OncoTargets and therapy

Article Title: Dynamic changes to survivin subcellular localization are initiated by DNA damage

doi:

Figure Lengend Snippet: UV radiation repartitions survivin from the cytosol to the mitochondria and nucleus. M059K (DNA-PK +/+ ), M059J (DNA-PK −/− ), GM02184E (ATM +/+ ), GM03189D (ATM −/− ), GM847 (ATR +/+ ) and GM847Kd (ATR kinase dead), exposed to 50 or 100 J/m 2 UV for 24 hours were subcellularly fractionated into cytosol ( A ), mitochondria ( B ) and nuclear ( C ) fractions and analyzed by Western blotting.

Article Snippet: Osteosarcoma (SaOS2), breast carcinoma (MCF7) cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA) while M059K (DNA-PK +/+ ), M059J (DNA-PK −/− ), GM02184E (ATM +/+ ), GM03189D (ATM −/− ) cells were obtained from the Coriell Institute (Camden, NJ).

Techniques: Western Blot